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KMID : 0354619920040010265
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Journal Dankook Dental Research Institute
1992 Volume.4 No. 1 p.265 ~ p.274
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Effects of Carbonic Anhydrase Inhibitors on the LPS-lnduced Bone Resorption In Vitro
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Abstract
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To study bone resorption mechanism, effect of LPS on the 45Ca release from fetal rat ulnae and radii, and effects of carbonic anhydrase inhibitors on the LPS-induced bone resorption in organ culture were studied. μCl 45CaC2 into their mother on
the
17th day of gestation. Radioactivities of 45Ca release into media were determined after 24, 48 and 72 hours, Effects of LPS and carbonic anhydrase inhibitors were observed by the ratio of % release of 45Ca between paired control and experimental
group.
@ES The observed results were as follows :
@EN 1. LPS(1㎍/ml) supplemented in media fior 72hours increased the 45Ca release significantly after 48 and 72 hours of culture and LPS(10㎍/ml) increased the 45Ca release significantly after 72 hours of culture.
2. LPS-induced 45Ca release was not inhibited significantly by 1mM sulfanilamide but inhibited significantly by 10mM sulfanilamide after 48 and 72 hours of culture.
3. LPS-induced 45Ca release was not inhibited significantly by 0.1mM dichlorphenamide but inhibited significantly by 1mM dichlorphenamide after 48 and 72 hours of culture.
4. LPS-induced 45Ca release was no inhibited significantly by 1mM acetazolamide but inhibited significantly by 5mM acetazolamide after 72 hours of culture.
Ulnae and radii were removed from 19 day old fetal rats, prelabelled by subcutaneous injection of 200
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