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KMID : 0354619940060010165
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Journal Dankook Dental Research Institute
1994 Volume.6 No. 1 p.165 ~ p.173
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HISTOPATHLOGIC STUDY ON DMBA INDUCED CARCINOGENESIS OF HAMSTER BUCCAL POUCH
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Abstract
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After authors used 2-3 months, 150-200gm, male Golden Syrian Hamster 25 and divided control 10, experimental 15, these animals were painted with 0.5% DMBA(9,10-dimethyl-1,2-bezanthracene) 3 times weekly for 12weeks. According to groups, these animals were sacrificed with at 2,6,8,12,16 weeks and were fixed in Garny´s solution fof 2 or 3 hours. And paraffin sections were stained with hematoxyline and eosin, which were examined with light micorscope. The results were as follows.
1. Hstopathogic findings of 2 week showed mild basilar hyperplasia, inflammatory cell infiltration, acnthosis, hyperchromatism, and moderate keratin and granular cell layer hyperplasia.
2. Hstopathogic findings from 6 to 8 week showed moderate basilar hyperplasia, inflammatory cell infiltration, acnthosis, and severe keratin and granular cell layer hyperplasia, which was considered as leukoplakia.
3. Histepathogic findings of 12 week showed a decreased keratin and granular cell layer hyperplasia, and prominantly increased hyperchromatism, pleomorphlism, mitosis and loss of polarity in basal cell layer, and prominant nucleoli, which was considered as carcinoma in situ.
4. Histopathogic findings of 16 week showed mainly exrophytic growth, and invasive dysplastic cells, and keratin formation as cancer pearl, which was well differentiated squamous cell carcinoma.
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KEYWORD
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