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KMID : 0354619940060020261
Journal Dankook Dental Research Institute
1994 Volume.6 No. 2 p.261 ~ p.270
Electromicroscopic Study of Submandibular Gland cell Development in Postnatal Rat



Abstract
The development and proliferation of submandibular gland cells in rat occur after birth. In the early stages after birth, typical acinar cell or granular duct can't be seen but terminal tuble cell can be found in the end portion of the
submandibular
gland of rats.
In this study, Sprauge dawley rats were sacrificed at 5, 10, 20 and 28 days after birth an the submandibular glands were sectioned. One group was fixed in 10% neutral formalin. 4§­ paraffin sections were stained with Hematoxilin-Eosin and
observed
under
light microscope. The other group was fixed with 2.5% glutaraldehyde, stained with uranylacetate and lead citrate and observed under an electronic microscope.
@ES The results were as follows.
@EN 1. In postnatal 10 days microvilli could be seen in the intercalated duct, and beyondpostnatal 20 days, the size & number of microvilli decreased with time in the terminal tuble cell.
2. After 20 days, fusion of excretory granules in the acinar cell increased. There were nearly no mitochondrias, and a small accaunt of rER could be seen.
3. On the 5 th day acinar cell differentation from proacinar cell was greater than that from terminal tuble cell.
4. Beyond postnatal 10 days, Acinar cell was differentated from terminal tuble cell because of secretory granule decrease in the terminal tuble cell until the 28th day, termmal tuble cells differentiated into the intercalated duct.
From the previous results. If could be seen that in the early stages after birth, acinar cell differentation from proacinar cell was more active than from terminal tubule cell from the 5th to the 10th day, and until the 28th day terminal tuble
cell
differented into the intercalated duct could be seen.
KEYWORD
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