|
KMID : 0354619950070010001
|
|
Journal Dankook Dental Research Institute
1995 Volume.7 No. 1 p.1 ~ p.20
|
|
|
PROLIFERATION OF SUBMAXILLARY GLANDULAR CELL IN ISOPROTERENOL TREATED RATS ON POSTNATAL PERIOD:IMMUNOHISTOCHEMICAL EVALUATIONS
|
|
|
|
|
Abstract
|
|
|
Isoproterenol,β-adrenergic drug, induce the stimulation of DNA synthesis of parotid and subrnaxillary gland by single injectionand prorrdnant hyperplastic and hypertrophic enlargement by multiple injection.
The purpose of this study was to investigate the proliferation, and differentiation of submaxillary gland of new born rat from IPR treated pregnant rats in order to understand the developemont and cellular proliferation of salivary gland.
150-200 gm, female Sprauge Dawley rats were mated and divided into two groups as control and experimental group. Experimental group were injected at same time on 15, 16, 17 days after pregnancy with IPR 16ug/g through intraperiuneum. 5 new born rats were sacrificed at 2, 3, 5, 7, 11, 14, 21, 28 days and 2 submaxillary glands were fixed in Bouni´s solution for 8-12 hours. 4um paraffin sections with pol-L-lysin slide were stained with H&E. In order to examine cellular proliferation and differentiation, immunohistochemical staining was done. Anti-PCNA antibody and Anti-EGF antibody as primary antibody, and Rabbit anti- mouse biotinylated IgG and Goat anti-rabbit biotinylated IgG as secon- dary antibody were used with ABC method. DAB as chromogen and balsam mounting were done. PCNA positive cells of submaxillary gland were counted at 3 different sites under the light microscope of 200 power field. According to the obtained numbers, they were divided into 20 stages and compared with those of control group. EGF positive cells were divided inte 5 stages according to their stainability. The results were as fellows.
1. Body weight of rat in experimental group markedly decreased after postnatal 21 days than that of control group while gland weight was 3-3.8 times heavier within postnatal 7 days.
2. Histopathologic features showed mainly proacinar and terminal tubule cells in early stage, while proacinar cells disappeared after postnatal 14 days, and terminal tubule cells markedly decreased but acinar cells prominantly increased.
3. Immunohistochemical features of PCNA showed active proliferation of proacinar cells in postnatal 2day of experimental group, which were subsided, while acinar cells presented the most active proliferation in postnatal 7 days, and proliferation of terminal tubule cells were to be continued, Proliferation of intercalated and striated duct cells was relatively to be continued.
4. Immunohistochemical features of EGF showed moderate positive mucous cells after postnatat 7 days, and moderate positive terminal tubule cells from postnatal 5 to 14 days. Intercalated duct and striated duct cells showed moderate positivity after postnatal 14 days, and strong on 38 days.
From the above mentions, the increased PCNA positivity in postnal 2-7 days strongly suggested transformation of proacinar cell into acinar cell.
Among the glandular cells, acinar ina terminal tLlbule cells continously showed proliferative activity afterpostnatal 7 days. EGf positivity of mucous, inter calated duct and striated duct cells were simitar io those of control group after postnatal 14 days. It might mean that WR had little effect on EGF stainability.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|