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KMID : 0354619960080010011
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Journal Dankook Dental Research Institute
1996 Volume.8 No. 1 p.11 ~ p.24
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Experimental Study on the Bone Healing Effects of Estrogen on Osteoporosis in Ovariectomized Rats
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Abstract
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Osteoporosis is a state of reduced bone mass per unit volume with a normal ratio of mineral to matrix, and induce fracture easily. The etiology of osteoporosis are aging, insufficiency of calcium intake, deficiency of vit-D and estrogen et al..
The
important contributing cause of bony loss are known to decreased of estrogen after menopausal state. Therefore, the prevention and treatment methods of osteoporosis using estrogen have been studied.
The purpose of this study was to observe the healing effects of estrogen on bony deffect of osteoporotic rats.
In this study, osteoporosis was induced by bilateral ovariectomy. After 60 days, bony defect was forme constantly size in right femur. Experimental group was injected estrogen simultaneously after formation of bony deffect every week, and control
group
was not injected. And both group were sacrificed at 1, 2, 3, 4, 6 week and femur were harvested. 4mm paraffin sections were stained with Hematoxyline-Eosin staining and immunohistochemical staining with PCNA antibody, and observed light
microscope.
@ES The results were as follows,
@EN 1. In control group, cartilagenous tissues was seen from 3 weeks, and lasted to 6 weeks. Formation of new bone was seen.
2. In experimental group, differentiation of osteoblasts was seen from 1 week, and formation of
ormal bony trabecule was seen in comparison with control group.
3. PCNA positive cells could be seen from 2 to 6 weeks in control group, but in experimental group, active PCNA positive cells could be seen at 1 week and decreased form 3 weeks.
From the above findings, we could concluded that there were activation of osteolbasts at early stage and after 4 weeks, formation of bony trabecule and calcification in experimental group.
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KEYWORD
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