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KMID : 0355420000240030299
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Journal of Korean Academy of Oral Health
2000 Volume.24 No. 3 p.299 ~ p.307
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The study of mass production of glucanase from Streptomyces sp. Y9343 for hydrolysis of dental plaque
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Yoon Jeong-Weon
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Abstract
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For the development of novel dental supplies could by mass production of dental plaque degradable enzyme.
The purpose of study was to investigate the optimal condition of mass production of glucanase from Steptomyces sp. Y934, its produced high activity of dental plaque degradable glucanase. Our study was incresed of glucanase secretion from Streptomyces sp. Y9343 by different carbon source and its addition time after 3 days growth of the culture.
The obtained results were is as follows
1. For the purpose of mass production of glucanase from Streptomyces sp. Y9343 used insoluble glucan(from Streptococcus mutans), dextrin(from corn), dextran(from Leuconostoc mesenteroides) into culture medium, 1% dextrin produced high glucanase among. them. Further culture medium used dextrin because it´s economic carbon source.
2. An electron micrograph, the shape of spores are smooth and round surface, and rectiflexible chain of spores.
3. Glucanase production was increased in the stationary phase of 0.5% starch medium in which added 1% dextrin: after 3 days.
This result implies that the production of glucanase influence adding time of dextrin as inducer.
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