KMID : 0358220060330020105
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Korean Journal of Fertility and Sterility 2006 Volume.33 No. 2 p.105 ~ p.113
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Role of ERK (Extracellular Signal Regulated Kinas) and PPAR¥ã (Peroxisome Proliferator-Activated Receptor Gamma) on TGF-¥â1 Induced Human Endometrial Stromal Cell Decidualization
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Chang Hye-Jin
Lee Jae-Hoon Kim Mi-Ran Hwang Kyoung-Joo Park Dong-Uk Min Churl-K.
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Abstract
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Objective: To investigate the role of ERK and PPAR¥ã on the TGF-¥â1 induced human endometrial stromal cell decidualization in vitro.
Method: Endometrial stromal cells are cultured under the following condition: DMEM/F12 (10% FBS, 1 nM E2 and 100 nM P4). TGF-¥â1 (5 ng/ml), Rosiglitazone (50 nM), and PD98059 (20 ¥ìM) were added according to experimental purposes. Trypan-Blue and hematocytometer were utilized to count cell number. Enzyme-linked immunosorbent assay (ELISA) and western blotting were utilized to detect proteins.
Result: TGF-¥â1 inhibited proliferation of cultured human endometrial stromal cells and induced expression of PGE2 and prolactin. This effect was mediated by Smad and ERK activation. Administration of rosiglitazone, PPAR¥ã agonist,prevented TGF-¥â1 effect on cell proliferation. Furthermore, Rosiglitazone inhibited TGF-¥â1 induced activation of ERK, consequently reduced PGE2 and prolactin production.
Conclusion: TGF-¥â1 induced decidualization of endometrial stromal cell through Smad and ERK phosphorylation.
PPAR¥ã acts as a negative regulator of human ndometrial cell decidualization in vitro.
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KEYWORD
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Decidualization, TGF-¥â1, PPAR¥ã, ERK, Smad
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