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KMID : 0359319690090010125
Korean Journal of Veterinary Research
1969 Volume.9 No. 1 p.125 ~ p.146
Studies on the Biological Assay of Black leg Vaccine and Antiserum

Abstract
Throughout the studies the following experimental results were summarized.
1. It was impossible to infect and kill the mice, weighing 10 to 12 gm, by inoculating 0.2㎖ of virulent Cl. chauvoei, diluted 1 to 10 with physiological saline, via subcutaneous, intramuscular, intraperitoneal or intraveonus, route.
2. The mice which were inoculated in brain with 0.03㎖ of Cl. chauvoei diluted 1 : 5120 with physiological saline were resulted in all death after infection, but not in case of attenuated strain even in dilution of one to five.
3. Virulent Cl. chauvoei were diluted with each of those of whole blood, erythrocytes and serum of horse, calf, swine, sheep, rabbit, guinea pig, chicken and duck, human plasma and 2% CaCl solution, and inoculated subcutaneously 0.25 to 0.5㎖ in mice, weighing 12 to 15gm. It was resulted in significant increase in virulence as comparing with the case of physiological saline solution except when horse and pig sera were used. Such a phenomena were not seen in attenuated strain.
4. Virulence of virulent Cl. Chauvoei could be increased significantly in rat, as the procedures used in mice, by suspending in whole blood, erythrocytes, serum, or plasma of various animals, or 2% CaCl₂ solution and by inoculating subcutaneously 0.5 to 10㎖ in rat, weighing 30 to 60 gm, as compared with those of control group which used physiological saline solutionos diluent.
5. Mice resisted 100 and 80 percent against challenge of 10³ and 10⁴ M. L. D.. respectively, 24 hours after inoculation of 0.5㎖ black leg antiserum.
6. Immune response to the black leg living vaccine in mice could be obtained more favorably in the group of respected vaccination rather than those of single inoculation and the most profitable inoculm size of the vacine was 0.5 to 1.0㎖.
7. Challenge for the immunized mice could be carried out effectively 3 weeks after first vaccination.
8. Satisfactory results could be obtained by inoculating subcutaneously for the immunization and intracerebrally or subcutaneously for the challenge.
9. Mice which were inoculated with 0.5㎖ of black leg living vaccine via subtaneucously two times at seven days interval and 21 days after first inoculation and challenged with 5 and 10 M. L.D. of virulent strain, resited 100 and 70 to 80 percent respectively. Same results were obtainable in black leg killed vaccine as the procedures used in living vaccine.
10. There were significantly different resistances against the definite challenge does between the mice groups which were immnuized with the living vaccine diluted five or
10 times and the undiluted.
11. For the biological assay of black leg living vaccine and antiserum, satisfactory results could be obtained using mice.
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학술진흥재단(KCI)