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KMID : 0359319740140020151
Korean Journal of Veterinary Research
1974 Volume.14 No. 2 p.151 ~ p.157


Abstract
This study was carried out to investigate on the morphology, distribution and stainability of the mast cells in the Korean Native goat. For the study, the experimental animals were anesthetized with pentobarbital sodium and opened the anterior abdomianl wall to remove immediately the specimens with a minimum of mechanical effects. The mesenteries were fixed in 10% neutral formalin, 4% basic lead acetate, absolute alcohol and ethlene glyco1 monoethyl ether. Following 24 hours of fixation, the toto preparation stained with 0.4% toluidine blue, 1% methylene blue, 1.5% bismak brown, saturated thionine and thionlne-methylene blue complex solution. The preparation were observed from 10 microscopic field with 450 magnification.
The results were as follows:
1. The form of the mesenteric mast cell was found 2 types. One was spindle form in larger number around vessels, the other was ovoid or spherical form in connective tissue far from blood vessels .
2. The average size was 18.63±5.75㎛ in length, 10.61±3.39㎛ in width and number was 105.50± 18.45.
3. Ethylene glycol monoethyl ether was particularly useful in preserving the mast cell granules.
4. Thione-methylene blue complex solution might be recommended to stain of granules.
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학술진흥재단(KCI)