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KMID : 0359319860260010097
Korean Journal of Veterinary Research
1986 Volume.26 No. 1 p.97 ~ p.102
Studies on Development of Escherichia coli Subunit Vaccine against Calf Diarrhea
김종만/Kim, Jong Man
김봉환/박정문/윤용덕/Kim, Bong Hwan/Park, Jeung Moon/Yoon, Young Dhuk
Abstract
The prevalence of diarrhea caused by enterotoxigenic Escherichia coli was surveyed on 445 calves in 6 farms which were located in the central part of Korea.
The incidence of enterotoxigenic Escherichia coli in calves with diarrhea was investigated by detecting the K99 and F41 antigens from the isolated strains of Escherichia coli
The incidence of colibacillosis in calves was 23.3%. Of 238 strains of Escherichia coli isolated from calves with diarrhea, 73 strains(30.6%) were proved possessing the K99 antigen by mannose-resistant hemagglutination(MRHA) using horse red blood cells and 79(33.1%) possessing F41 antigens by MRHA using guinea-pig red blood cells.
The minea medium, nutrient broth, tryptic soy broth and brain heart infusion were tested for yield of K99 and F41 pili. The production of pili was greatest in minea medium.
The best detachment method of the K99 and F41 pili from the cells was heat treatment for 20 minutes at 60℃ and concentration by precipitation with ammonium sulfate.
The purified antigens of K99 and F41 were polypeptides with molecular weights of 18,500 and 29,500, respectively by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
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