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KMID : 0359319910310010049
Korean Journal of Veterinary Research
1991 Volume.31 No. 1 p.49 ~ p.53
Phytomitogen induced blast tansformation of rabbit


Abstract
The present study has been carried out to investigate the optimal condition on lymphocyte blastogenesis of rabbit lymphocytes, whole blood culture and microculture system in conjunction with a semiautomatic multiple sample harvester(SAMSH) was used to study the in vitro optimal condition of rabbit lymphocytes.
Data were presented to show many variables that are involved in studying the phytohemagglutinin(PHA) and lipopolysaccharide(LPS) response of rabbit lymphocyte in a microculture system.
Analysis indicated that the conditions for optimal PHA as measured by incorporation of ³H-TdR include:
(1) use of RPMI-1640 as culture medium.
(2) use of 6㎍ of PHA, per culture.
(3) 48-hours culture period.
Conditions for optimal stimulation with LPS mitogen were similar to those used for PHA.
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학술진흥재단(KCI)