KMID : 0359319930330040711
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Korean Journal of Veterinary Research 1993 Volume.33 No. 4 p.711 ~ p.717
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Serum cortisol concentrations on the normal condition in Korean native goats by ELISA
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Abstract
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This study was performed to examine the charges of serum cortisol concentrations on the normal conditions in Korean native goats by enzyme-linked immunosorbent assay(ELISA).
The Korean native goats were 4 to 30 months old and weighted 6.0 to 28§¸. The goats were allowed ad libitum access to water and food.
To minimize the stress with handing and blood sampling, animals were adapted for 2 weeks before the experiment. After adaptation, intravenouse catheter was inserted left intact a jugular vein of 6 goats and blood samples were done 2¡3 days later. Experimental animals were divided into 2 groups which was non-catheterized and catheterized group in order to examine the effect for changes of serum concentration and circadian rhythm of cortisol.
The results were obtained as follows ;
The sensitivity of serum cortisol concentration was 20 pg/§¢. The intra-assay and inter-assay coefficient of variation were below 2.5% and 5%, respectively.
Serum concentration of cortisol was more higher in noo-catheterized group(32.84¡¾16.78 ng/§¢) than catheterized group(23.20¡¾10.29 ng/§¢). The difference according to months old in serum concentration of cortisol was the more higber on 4¡6 than 7¡12 and lowest over 12 months old.
A circadian rhythm in the serum concentration of cortisol in Korean native goats was not found significantly with 2 hours sampling intervals.
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