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KMID : 0359319950350040769
Korean Journal of Veterinary Research
1995 Volume.35 No. 4 p.769 ~ p.776
Production of monoclonal antibody(MAb) for lymphocytes of Korean native cattle 1. MAb to T lympho
Song Hee-Jong

Woon Jae-Ho
Kim Tae-Joong
Chai Hyo-Seok
Hur Boo-Hong
Chin Min-Soon
Abstract
This study was undertaken to develop the monoclonal antibody(MAb) for lymphocytes of Korean native cattle by the cell hybridization of myeloma P3/NS-1/ 1-Ag-4-1 and spleen cells from BALB/c mice hyperimmunized with nylon wool column eluted peripheral T lymphocytes of Korean native cattle. The isotype of MAb KCT-14 against T lymphocyte was mouse IgG1 KCT-14 positivity of mononuclear cells(MNC) from peripheral blood lymphocytes, nylon wool nonadherent and adherent-lymphocytes was 41.7%, 58.4% and 22.6%, respectively. And that of mesenteric lymph node-, spleen and thymus-MNC was 43.3%, 40.2% and 33.6%, respectively. Immunoperoxidase staining of frozen tissue sections showed that the MAb positive cells were located in the medulla of the thymus and in the paracortical area and the mantle zone of the germinal center in the lymph nodes. These results indicated that KCT-14 was one of the MAb for investigate of T lymphocyte subpopulations in the Korean native cattle.
KEYWORD
monoclonal antibody , T lymphocytes , Korean cattle , ELISA , immunoperoxidase
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학술진흥재단(KCI)