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KMID : 0359320020420040513
Korean Journal of Veterinary Research
2002 Volume.42 No. 4 p.513 ~ p.522
Detection of Salmonella spp. By TaqMan real-time PCR and comparison of nucleotide sequences of ompC gene among Salmonella
Lee Young-Sung

Choi Kyoung-Seong
Kim Myeong-Chul
Han Jae-Cheol
Chae Joon-Seok
Abstract
Antigenic ompC henes of S. gallinarum, S. pullorum and S. dublin were characterized among Salmonella spp. isolated from chickens and other animals to identigy genetic variation. Salmonella ompC gene fragment (1.027 bp) was amplified by PCR and the amplicons were cloned for comparison of nucleotide sequences. The identity of the sequences between S. gallinarum and S. pullorum, S. gallinarum and S. dublin, S. pulloru, and S. dublin was 99.8%, 97.6% and 97.8%, respectively. Also, we found that ompC has some diversity between S. gallinarum and S. pulloru, and other Salmonella spp. which may be useful to type the ofganisms. Similar to diagnosis in other organisms, the TaqMan PCR method can be applied to rapid and accurate diagnosis of salmonellosis in chickens and other animals. We designed PCR primers and TaqMan probe for flagellin gene (fliC) for detection of Salmonella spp. by TaqMan PCR. The TaqMan PCR method was 10,000 times more sensitive than conventional PCR.
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학술진흥재단(KCI)