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KMID : 0359320130530010025
Korean Journal of Veterinary Research
2013 Volume.53 No. 1 p.25 ~ p.28
Application of SYBR Green real-time PCR assay for the specific detection of Salmonella spp.
Shin Seung-Won

Cha Seung-Bin
Lee Won-Jung
Shin Min-Kyoung
Jung Myung-Hwan
Yoo An-Na
Jung Byeng-Yeal
Yoo Han-Sang
Abstract
The aim of this study was to applicate and evaluate a SYBR Green real-time PCR for the specific detection of Salmonella spp. Specificity of the PCR method was confirmed with 48 Salmonella spp. and 5 non-Salmonella strains using invA gene primer. The average threshold cycle (CT) of Salmonella spp. was 11.83 ± 0.78 while non-Salmonella spp. was 30.86 ± 1.19. Correlation coefficients of standard curves constructed using CT versus copy number of Salmonella Enteritidis ATCC 13076 showed good linearity (R2 = 0.993; slope = 3.563). Minimum level of detection with the method was > 102 colony forming units (CFU)/mL. These results suggested that the SYBR Green real-time PCR might be applicable for the specific detection of Salmonella spp. isolates.
KEYWORD
invA, Salmonella spp, specific detection, SYBR Green real-time PCR
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학술진흥재단(KCI)