KMID : 0359320170570010037
|
|
Korean Journal of Veterinary Research 2017 Volume.57 No. 1 p.37 ~ p.42
|
|
Establishment of reverse transcription polymerase chain reaction for detection of Getah virus infection in livestock
|
|
Lee Seung-Heon
Yang Dong-Kun Kim Ha-Hyun Choi Sung-Suk Cho In-Soo
|
|
Abstract
|
|
|
Getah virus (GETV) infection causes sporadic outbreaks of mild febrile illness in horses and reproductive failure in pigs. In this study, we established a reverse transcription polymerase chain reaction (RT-PCR) method to detect GETV from suspected virus-infected samples. The reaction conditions were optimized and validated by using RNA extracted from GETV propagated in cell culture. A GETV-specific GED4 primer set was designed and used to amplify a 177 bp DNA fragment from a highly conserved region of the E1 glycoprotein gene in the GETV genome. RT-PCR performed with this primer set revealed high sensitivity and specificity. In the sensitivity test, the GED4 primer set detected GETV RNA at the level of 102.0 TCID50/mL. In the specificity test, the GED4 primer set amplified only a single band of PCR product on the GETV RNA template, without non-specific amplification, and exhibited no crossreactivity with other viral RNAs. These results suggest that this newly established RT-PCR method is useful for accurate identification of GETV infection in animals.
|
|
KEYWORD
|
|
Getah virus, diagnosis, reverse transcription polymerase chain reaction
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|