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KMID : 0359320180580040211
Korean Journal of Veterinary Research
2018 Volume.58 No. 4 p.211 ~ p.217
Differentiation potential of canine mesenchymal stem cells on hydrogel scaffold-based three-dimensional environment
Gu Na-Yeon

Park Mi-Jeong
Lee Jienny
Byeon Jeong-Su
Jeong Da-Un
Cho In-Soo
Cha Sang-Ho
Abstract
Mesenchymal stem cells (MSCs) are useful candidates for tissue engineering and cell therapy. Physiological cell environment not only connects cells to each other, but also connects cells to the extracellular matrix that provide mechanical support, thus exposing the entire cell surface and activating signaling pathways. Hydrogel is a polymeric material that swells in water and maintains a distinct 3-dimensional (3D) network structure by cross linking. In this study, we investigated the optimized cellular function for canine adipose tissue-derived MSCs (cAD-MSCs) using hydrogel. We observed that the expression levels of Ki67 and proliferating cell nuclear antigen, which are involved in cell proliferation and stemness, were increased in transwell-hydrogel (3D-TN) compared to the transwell-normal (TN). Also, transforming growth factor-β1 and SOX9, which are typical bone morphogenesis-inducing factors, were increased in 3D-TN compared to the TN. Collagen type II alpha 1, which is a chondrocyte-specific marker, was increased in 3D-TN compared to the TN. Osteocalcin, which is a osteocyte-specific marker, was increased in 3DTN compared to the TN. Collectively, preconditioning cAD-MSCs via 3D culture systems can enhance inherent secretory properties that may improve the potency and efficacy of MSCs-based therapies for bone regeneration process.
KEYWORD
chondrogenesis, extracellular matrix, hydrogels, mesenchymal stem cells, osteogenesis
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학술진흥재단(KCI)