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KMID : 03644199719970000287
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East-West Medical Research Institute Journal
1997 Volume.1997 No. 0 p.287 ~ p.296
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Effects of Ijingtang fractions on activities of NaK ATPase and Ca ATPase in rat brain synaptosome
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조영옥
한승호/김창주/민병일/이태희/윤상협/오수명
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Abstract
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To explore the action mechanism of Ijintang in the brain, the authors investigated the effects of Ijintang fractions on MgNaK ATPase and MgCa ATPase in rat brain synaptosomes prepared from cerebral cortex. The activities of MgNaK ATPase and MgCa ATPase were assayed by the level of inorganic phosphate liberated from the hydrolysis of ATP. Fraction WH-95-7 at the concentration Jf 10-2% decreased the activity of MgNaK ATPase about 34.1% and also reduced the activity of MgCa ATPase about 49.3%. But, other fractions (WB-95-7, WC-95-7, MB-95-7, MC-95-7, 1iH-95-7) did not significantly changed the activities of the MgNaK ATPase and MgCa ATPase. The decreased activity of MgNaK ATPase by WH-95-7 will decrease the rate of Cat´ efflux, Probably via an Na-Ca exchange mechanism and will increase the rate of Cat´ entry by the depolarization of nerve terminals. The reduced activity of MgCa ATPase by WH-95-7 will result in the decreased efflux of Cal+. As a conclusion, it can be speculated that lithium elevates the Intrasynaptosomal Ca 2+ concentration via inhibition of the activities of MgNaK ATPase and MgCa
ATPase, and this increased [Ca´+]i will cause the release of neurotransmitters.
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