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KMID : 03644199819980000149
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East-West Medical Research Institute Journal
1998 Volume.1998 No. 0 p.149 ~ p.150
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The Effect of Injinchunggantang-derivative on Proliferation of Hepatocyte
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Abstract
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The purpose of this study is to evaluate the effect of Injinchunggantang-derivative on 31iferation of hepatocyte in rats. Cell viability is studied by MTT assay. The gene related to cell ?lication such as p53, wafl, bc1-2 and bcl-XL is quantitized by quantitative RT-PCR and the ateins coded by these genes are studied by Western blotting. The results are as follows.
1. The hepatocytes cultured in medium with Injinchunggantang-derivative showed better viability mpared with control group in MTT assay, and the hepatocytes cultured in medium with the jinchunggantang-derivative-and-ethanol-mined group showed better viability than the hepatocytes Itrued in 10%´ ethanol culture medium(control group), noting that Injinchunggantang-derivative ts protective effect on hepatocyte injury. There was no dose- and time-dependence.
2. In quantitative RT-PCR,
i) Bcl-2- ´gene increased significantly both in Injinchunggantang-derivative group and in jinchunggantang -derivative-and-ethanol -mixed group, while it showed no significant increase or crease in other group.
ii) I3c1-xL gene increased significantly in Injinchunggantang-derivative group as well as in jinchunggantang-derivative-and-ethanol-mixed group.
iii) P53 gene showed no significant increase or decrease in hepatocytes cultured in medium with 10% ethanol and in hepatocytes cultured in medium with Injinchunggantang-derivativemd-ethanol-mixed group, suggesting that 10% ethanol induced cell toxicity, thus increased p53 ;ene expression.
iv) Wafl gene showed no significant increase or decrease in hepatocytes cutured in medium with Inj inchunggantang -derivative, while increased in hepatocytes cultured in medium with 10% ethanol and in hepatocytes cultured in medium with Injinchunggantang-derivative-andethanol-mixed group, suggesting that 10% ethanol induced cell toxicity increased wafl gene expression.
3. In the study on protein by western blotting, the band of bcl-2 and bcl-XL were widened in Inj inchunggantang -derivative group. Especially the amount of bcl-x,. increased significantly compared with other groups. But in the study on p53 and wafl, there was no significant difference among those groups.
Above study shows that Injinchunggantang-derivative has good effect on cell viability and that the genes resistant to cell death such as bcl-2 and bcl-XL are induced by Injinchunggantang-derivative to resist to cell death by toxic agent. And this is reconfirmed in protein study using western blotting. These results suggest that Injinchunggantang -derivative has inhibitory effect on cell death as well as protective effect on hepatocyte. Therefore this prescription is recommended in various liver diseases such as chronic liver disease and drug-induced hepatic iniurv.
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KEYWORD
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