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KMID : 0366519950150010051
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Annual Bulletin Seoul Health
1995 Volume.15 No. 1 p.51 ~ p.72
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The Studies on Isolation of Actinomycetes and Discomposi tion of PAHS(RBBR dye) by Isolated Actinomycetes in Korean Soils
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Kim Tae-Jeon
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Abstract
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Decolorization of remazol brilliant blue R(RBBR)dye, which can take the place of the degradation of polycyclic aromatic hydorcarbons(PAHS) by actinomycetes isolated in 67 soil samples collected at 8 areas of Korea, and parameters that may influence decolorization were examined from February, 1994 to March, 1995. The results were summarized as following. ① Each actinomycetes isolated on Al medium, A2 medium and A3 medium from 67 soil samples were 822 strains, 763 strains, and 783 strains. therefore Al selective medium was very good for isolation of actinomycetes ② The optimal pH of soil samples for growth of actinomycetes was 7.0∼7.5. ③ The optimal temperature for degradation of lignocellulose, lignin, and decoloization of RBBR(0.005%)dye was 30℃. ④ Strains screened in A1S medium for the degradation of lignocellulose were 205 strains among 822 strains isolated in Al medium, strains screened in M3 IND medium for degradation of lignin were 108 strains among 205 strains, and 108 strains were identified finally with one strain of amycolata autotrophica and 13 strains of streptomyces. Strains indicated decolorization ratios over 50% by agar plug and standing culture were 17 strains among 108 strains, and strains showed decolorization ratios over 60% by cell suspension (O.D; 0.85, inoculation volume: 0.2ml) and shaking culture were 12 strains among 17 strains. ⑤ Decolorization ratios of 12 strains according to carbon sources(0.2%) were very good in glycerin with 73.2% to streptomyces halstedii scabies SAl-36 and in cellobiose with 73.1% to streptomyces lavendulas SA2-14. ⑥ Decolorization ratios according to the concentrations of cellobiose to streptomyces lavendulas SA2-14 and of glycerin to streptomyces halstedii scabies SAl-36 were the most high ratios with 74.1% in 0.25% cellobiose and with 73.4% in 0.25% glycerin. In the above results, it will be possible in the future to screen actinomycetes which can degrade PAH compounds included in natural, industrial wastes and stain solution discharged during to stain tissue preparations through decolorization abillity of RBBR dye by actinomycetes.
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KEYWORD
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Actinomycetes, Streptomyces, RBBR(Rimazol brilliant blue R) dye, PAHS(Polycyclic aromatic hydrocarbons), Decolorization, Discompsition
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