KMID : 0366519980180010193
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Annual Bulletin Seoul Health 1998 Volume.18 No. 1 p.193 ~ p.199
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Mutagenicity of Solid Waste Landfill Leachate
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Yoo Young-Sik
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Abstract
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Mutagenic activity was examined on leachate of Nanjido solid waste landfill, Kimpo landfill and Hwasung wastes treatment effluents by means of the Ames Salmonella microsome assay. Three concentration methods ; XAD resin adsorption, dichloromethane solvent extraction, and blue-rayon adsorption method were compared for their abilities to concentrate mutagens from a leachate sample obtained from Nanjido solid waste landfill. Dichloromethane solvent extracts well recovered mutagens from leachate sample among 3 concentration methods, while the blue-rayon adsorption could scarcely recover mutagens, suggesting that polynuclear aromatic hydrocarbons having 3 or more fused aromatic rings in their structures less contribute to the mutagenic activity of landfill leachate. Mutagenicity of Nanjido landfill leachate was higher(740¡1280 net revertants/L) than that of effluents from Kimpo and Hwasung(380¡820 net revertants/L). TA 98 strain detected mutagenicity of concentrates better than TA 100 strain and the addition of S-9 did not increase the number of revertants. It means that mutagens of frameshift and/or direct acting type are major components in leachate. Judging from these results, TA 98 strain without S-9 test system is suitable for detecting mutagenicity from waste landfill leachate.
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KEYWORD
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Landfill, leachate, effluent, dichloromethane extraction, mutagenicity
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