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KMID : 0366519980180010193
Annual Bulletin Seoul Health
1998 Volume.18 No. 1 p.193 ~ p.199
Mutagenicity of Solid Waste Landfill Leachate
Yoo Young-Sik

Abstract
Mutagenic activity was examined on leachate of Nanjido solid waste landfill, Kimpo landfill and Hwasung wastes treatment effluents by means of the Ames Salmonella microsome assay. Three concentration methods ; XAD resin adsorption, dichloromethane solvent extraction, and blue-rayon adsorption method were compared for their abilities to concentrate mutagens from a leachate sample obtained from Nanjido solid waste landfill. Dichloromethane solvent extracts well recovered mutagens from leachate sample among 3 concentration methods, while the blue-rayon adsorption could scarcely recover mutagens, suggesting that polynuclear aromatic hydrocarbons having 3 or more fused aromatic rings in their structures less contribute to the mutagenic activity of landfill leachate. Mutagenicity of Nanjido landfill leachate was higher(740¡­1280 net revertants/L) than that of effluents from Kimpo and Hwasung(380¡­820 net revertants/L). TA 98 strain detected mutagenicity of concentrates better than TA 100 strain and the addition of S-9 did not increase the number of revertants. It means that mutagens of frameshift and/or direct acting type are major components in leachate. Judging from these results, TA 98 strain without S-9 test system is suitable for detecting mutagenicity from waste landfill leachate.
KEYWORD
Landfill, leachate, effluent, dichloromethane extraction, mutagenicity
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