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KMID : 0615219780040010055
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Journal of Kwangju Health
1978 Volume.4 No. 1 p.55 ~ p.67
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Studies on the Production of Glutathione by Rhodotorula Glutinis -Cultural Condition of Extractrion and Purification of Glutathione
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성하진
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Abstract
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Results obtained from a series of experiments on cultural conditions of Rhodotorula glutinis for glutathione production, on extraction methods, and on purification of glutathione were as follows.
To maximize the glutathione content in the cell mass of R. glutinis, addition of Mg ion by 0.05% and Ca ion 0.01% to the basal medium was favorable.
The optimal concentration of methionine added to the medium was approximately 2.5×10^(-3)M.
Boiling water extraction at 90℃ for 15 minutes and ultrasonic extraction at 60 kc for 15 minutes were favorable method of extraction. Highest yield was obtained when extracted with ultrasonic treatment and subsquent boiling water treatment.
The final recovery rate after purification was increased from 49.3% to 57.1% by addition of sodium gluconate at a 0.02% level.
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