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KMID : 0615219860110010021
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Journal of Kwangju Health
1986 Volume.11 No. 1 p.21 ~ p.30
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Studies on the Protein Electrophoretic Patterns and Minerals during Metamorphosis of the German Cockroach, Blattella germanica L.
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吳世源/Oh SW
金東弼/Kim DP
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Abstract
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The protein electrophoretic patterns, molcular weight of protein and the concentrations of mineral salts during metamorphosis of the german cockroach, Blattella germanica L. were measured by using SDS-polyacrylamide gel electrophoresis and Atomic absorption spectrophotometric methods, respectively.
Healthy specimens were chosen as samples of each developmental stages; the second nymph, the fourth nymph, the sixth nymph and adult.
Protein patterns were shown to form 24 bands according to their mobilities on electrophoresis but qualitative differences manifested in their patterns among all stages.
At all developmental stages, the highest molecular weight was about 202,000 and the lowest one was about 17,000.
A low molecular weight band was diminished, whereas an optical density of the high molecular weight band was in high concentration at adult stage.
Mineral salts such as calcium, magnesium, zinc, iron, phosphorus, potassium and sodium were analyzed at all stages of metamorphosis.
Changes in zinc, potassium and sodium concentrations through the coures of the physiological cycle showed generally a U-shaped curve during metamorphosis.
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