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KMID : 0615219880130010017
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Journal of Kwangju Health
1988 Volume.13 No. 1 p.17 ~ p.27
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Immobilization of Pepsin on Chitosan Matrix
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李治榮
田成珠
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Abstract
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For the effective immobilization of pepsin on chitosan matrix with glutaraldehyde, optimal activation methods were studied, and its enzymatic properties were investigated.
In immobilizing pepsin activity experiments where it was immobilized on to chitosan matrix with glutaraldehyde, we found that the immobilized pepsin retained 65% activity.
The optimum pH of the soluble pepsin was 2.0. while immobilized pepsin was 1.5. The effect of temperature on the activity of the immobilized pepsin was compared with that of the native pepsin. The result indicated that the native pepsin was 35℃, while immobilized pepsin was most active over a temperature range from 35℃ to 65℃. From Line Weaver-Burk polts, the following kinetic data were abtained: for the immbolized pepsin, Km=0.417% and Vmax=0.769㎛/L:for the native pepsin, Km=0.476% and Vmax=1.333μM/L.
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