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KMID : 0615219910160010357
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Journal of Kwangju Health
1991 Volume.16 No. 1 p.357 ~ p.369
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Physiological, Biochemical and ultrastructural studies on Bleomycin induced nephrotoxicity in mice
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김상엽/Kim SY
조성태/Cho ST
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Abstract
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Bleomycin, a glycopeptide antibiotic isolated from Streptomyces Verticillus, ha been used as an anticancer drug active aganist human squamous cell carcinoma of skin and lung. In this experiment, the author pursed the effect of bleomycin on the mouse kidney, observing the physiological, biochemical and ultrastructural changes in glomerulus and convoluted tubules. Mice(ICR strain) were used as experiment animals which were divided into two groups, the control and experimental groups. In order to elucidate the nephrotoxicity of the mouse administrated bleomycin in D. W., this study was carried out. Bleomycin was injected 20 units per kg of body weight to abdominal portion of mouse in 1 week, 2 weeks, 3 weeks, and 4 weeks. After 5 weeks, the left kindey was taken out from the mouse with injicted bleomycin and investigatigated the damages of the cells by the ultrastructural inspections. The results obtained were as follows 1. The thickened basement mimbrane of glomerulus and closed urinary space, fused parietal epithelial cells were observed, respectively.
2. Destructed brush border, granular degeneration, formed crescent, fused foot process, vacuolation of proximal and distal convoluted tubules were shown.
3. Phsiological characteristics of experimental groups, thrombocyte, Hematocrit and MCV were significantly increased.
4. Total lipid concentration, blood urea nitrogen were slightly increased and total cholesterol and albumins were significantly increased on all experimental groups.
5. In the structural changes of the mitochondria in kidney treated with bleomycin the organelles changes into a spherical or oval shapes, irregular cristae and destructed mitochondria were shown.
From the above results, bleomycin damages both proximal and distal convoluted tubular epithelial cells and glomerulus in mice kidney.
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