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KMID : 0615219930180010317
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Journal of Kwangju Health
1993 Volume.18 No. 1 p.317 ~ p.327
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A Study on the isolation and restriction enzyme of DNA
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김상엽/Kim SY
박민희/Park MH
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Abstract
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The pBN18 of the gene fusion vector was double digestion with HindⅢ and EcoRⅠ for the analysis of the restriction enzyme sites.
As a results, the fragments of 1.2Kb and 4.6Kb were made. Therefore it was conformed that pBN18 includes the sites of HindⅢ and EcoRⅠ. The pBN18 contained the ClaⅠ site to digestion with ClaⅠ restriction enzyme.
A study was conformed the restriction enzyme sites of pBN18, pBN18A and pBN18B and DNA sizes of pBN18 and pBN18B were compared with the agarose gel of the electrophoresis.
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KEYWORD
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