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KMID : 0615219940190010187
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Journal of Kwangju Health
1994 Volume.19 No. 1 p.187 ~ p.201
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Production and characterization of monoclonal antibody to HIV-1
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이영종/Lee YJ
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Abstract
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In order to improve the reliability immunodiagnostic techniques being used in the diagnostic of HIV-1, monoclonal antibody was prepared and characterized. HIV-1 antigen was prepared and used as a immunizing antigen. Spleen cells of BALB/c mice immunized with HIV-1 were fused with myeloma cells.
Total number of fused cells obtained were 347 out of 384 seeded. A total of 124 fused cells were found to secrete antibodies to HIV-1 out of 347 fused cells. Among cells secreting antibodies, one well was performed limiting dilution. Total number of cloned cells obtained were 59 out of 196 seeded. A total of 24 cloned cells were founded to secrete antibodies to HIV-1 out of 59 cloned cells. among 24 cloned cells, only 5 cloned cells were performed western blot using HIV-1 western blot kit(Dupont). 2 monoclonal antibodies were found to react exclusively with p24 protein of HIV-1. By SDS-PAGE and EITB, the monoclonal antibody reacted HIV-1 p24 protein of 25,500+-500 dalton.
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KEYWORD
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