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KMID : 0615219940190010233
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Journal of Kwangju Health
1994 Volume.19 No. 1 p.233 ~ p.249
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Electron microscopic visualization of pBN18 plasmid DNA structure and expression in the Escherichia coli
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김상엽/Kim SY
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Abstract
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This study was visualized the transcriptional activity, the size and the modelof plasmid DNA by electron microscopy.
Plasmid pBN18, pBN18A and pBR322 were visualized by way of electron microscopy.
Mica-press adsorption method that alows selective visualization of the plasmid DNA released in situ from the bacterial cell is rapid and useful for visualization of plasmids.
The released plasmid DNA was adsorbed preferential on mica in a divalent cation-free solution, and the Miller chromatin-spreading method may allow the physical interaction and transcription to be visualized.
Therefore mica-press adsorption method and Miller chromatin-spreading method used to visualize the plasmid DNA, chromosomal DNA and gene expression.
As a result, their estimated sizes of plasmid pBN18, pBN18A, pBN18B and pBR322 were 1.87 0.28(5.6 Kb)?, 1.53 0.12(4.6Kb)?, and 1.50 0.03(4.50Kb)?, respectively, and transcriptional activity was observed by the visualization of gene expression of chromosomal DNA.
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KEYWORD
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