KMID : 0890520020040010046
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Korean Journal of Cerebrovascular Disease 2002 Volume.4 No. 1 p.46 ~ p.51
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Effect of Melatonin on Brain Polyamine Contents and Hippocampal Neuronal Damage after Transient Global Ischemia in Mongolian Gerbil
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Jang Young-Sik
Suh Young-Sung Lee Seung-Ryong Kim Dae-Hyun Yim Man-Bin
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Abstract
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Objectives: This study was designed to examine whether melatonin has a neuroprotective effect against hippocampal neuronal damage following transient global ischemia in a gerbil. Polyamine is known to play a role in the pathophysiology of ischemic neuronal damage, we evaluated the influences of melatonin on the polyamine level as well as histology.
Materials and Methods: Male Mongolian gerbils (60-80 g) were used in this study. Transient global ischemia was induced by occlusion of the bilateral common carotid arteries for 3 min with microclips. Melatonin was administered immediately after occlusion. The animals were decapitated 24 h after the occlusion for polyamine measurement by a high performance liquid chromatography (HPLC) and 4 days after the occlusion for histological evaluation (hematoxylin and eosin staining). A histological examination was performed by a blinded investigator.
Results: The hippocampal putrescine level increased compared to sham-operated animals and the increase of putrescine was attenuated by 20 mg/kg melatonin administration. Spermidine and spermine levels didn¡¯t show significant changes after ischemia. Hippocampal neuronal damage in the CA1 region was markedly observed in vehicle-treated animals compared to sham-operated animals. Melatonin administration (10 or 20 mg/kg) significantly inhibited hippocampal CA1 neuronal damage after ischemia compared to corresponding vehicle-treated animals (p<0.05 and p<0.01, respectively).
Conclusions: Melatonin attenuates the putrescine level after transient global ischemia and may have putative neuroprotective effects against global ischemia induced neuronal damage.
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KEYWORD
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Global ischemia, Gerbil, Polyamine, Hippocampus, Neuronal damage, Melatonin
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