KMID : 1039620150050000117
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Korean Journal of Family Practice 2015 Volume.5 No. 0 p.117 ~ p.121
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blaVIM-2 of Pseudomonas aeruginosa in primary hospitals in Korea
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Yoon Jeong-Sook
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Abstract
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Background : In 2007, the resistance rate of Pseudomonas aeruginosa to imipenem was 29% in Korean tertiary hospitals. The main cause of imipenem resistance is the metallo-¥â-lactamase. A few studies about metallo-¥â-lactamase are found in tertiary hospitals, however, there are few studies in primary hospitals. Therefore, we studied whether genes encoding metallo-¥â-lactamase were present in P. aeruginosa isolates from primary hospitals, and studied blaVIM-2, blaIMP-1, blaNDM-1 using molecular methods.
Materials and methods : A total of 960 blood, body fluid, open and closed pus, sputum, and urine specimens obtained from 27 primary hospitals of Bundang district were used this study. A screening test of metallo-¥â-lactamase was performed. The primers forblaVIM-2, blaIMP-1, andblaNDM-1 were used. DNA was extracted using the AccuPrep Nano-plus Plasmid mini Extraction Kit (Bioneer, Cheongwon, Korea). PCR was performed using AccuPower PCR PreMix (Bioneer).
Results : P. aeruginosa was isolated from 42 of the 960 specimens. After PCR was performed for all 42 isolates using the three sets of metallo-¥â-lactamase gene-specific primers, blaVIM-2 Was detected in one sputum isolate.
Conclusion : This study gives us warning that the metallo-¥â-lactamase could spread in the community.
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KEYWORD
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Pseudomonas aeruginosa, VIM-2, resistance
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