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KMID : 1129720000170010179
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Korean Journal of Acupuncture
2000 Volume.17 No. 1 p.179 ~ p.190
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The Effect of Juglandis Semen Extract Solution on Oxidant-Induced Alteration of Glutamate Uptake in Rabbit Brain Synaptosome
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Kim Tae-Kook
Yoon Hyun-Min
Jang Kyung-Jeon
Song Chun-Ho
Ahn Chang-Beohm
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Abstract
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This study was undertaken to determine whether Juglandis semen extract solution (JLS solution) exerts protective effect against oxidant-induced inhibition of glutamate uptake by synaptosomes. Synaptosome was prepared from rabbit brain cortex. Glutamate uptake increased by incubation time during 10 minutes, which was significantly inhibited by 1mM t-buthylhydroperoxide(t-BHP). JLS solution prevented t-BHP-induced inhibition of glutamate uptake in a dose-dependent manner. t-BHP reduced glutamate uptake in dose-dependent fashion, which was significantly prevented by 2% JLS solution. t-BHP(1mM) and ascorbate/Fe2+(50/1μM) increased lipid peroxidation in synaptosomes by 5-fold, and it was significantly prevented by 2% JLS solution. HgCl2(0.1mM) inhibited glutamate uptake and increased lipid peroxidation. These changes were prevented by 2% JLS solution. Synaptosomal Na-K-ATPase activity was inhibited by t-BHP(1mM) and H2O2(50mM), which was prevented by 2% JLS solution.The results indicate that JLS solution prevents oxidant-induced inhibition of glutamate by synaptosomes, and this may result from inhibition of lipid peroxidation induced by oxidants.
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KEYWORD
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Juglandis Semen, Oxidant, Glutamate uptake, Synaptosome, t-BHP
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