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KMID : 1129720010180010011
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Korean Journal of Acupuncture
2001 Volume.18 No. 1 p.11 ~ p.20
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Chemopreventive activity of Prunella Herba Vulgaris L. Aqua-acupuncture Solution
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Park Sin-Hwa
Lim Jong-Kook
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Abstract
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Cancer chemoprevention refer to the use of natural or synthetic substances to prevent the initiational and promtional events that occur during the process of carcinogenesis. The effect of Prunella Herba Vulgaris L. Aqua-acupuncture Solution (PVAS) and Prunella Herba Vulgaris L. Water-extracted Solution (PVWS) on the induction of phase Ⅱ detoxification enzyme (quinone reductase, Glutathione S-transferase) and inhibition of phase Ⅰ enzyme (cytochrome P4501A1) and benzo[a]pyrene-DNA adduct formation was examined. PVAS is potent inducers of quinone reductase activity. Glutathione levels were increased with PVAS, in cultured murine hepatoma Hepa1c1c7 cells. In addition glutathione S-transferase levels were increased with PVAS. However, there was 45.2% inhibition in the activity of cytochrome P4501A1 enzyme with the treatment of PVAS, 5×. At concentration of 1× and 3× of PVAS, the binding of [3H]B[a]P metabolites to DNA of NCTC-clone 1469 cell was inhibited by 25.3%, 45.0%, respectively. These results suggest that PVAS has chemopreventive potential by inducing quinone reductase and glutathione S-transferase activities, increasing GSH levels, inhibiting the activity of cytochrome P4501A1 and benzo[a]pyrene-DNA adduct formation.
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KEYWORD
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Prunella Herba Vulgaris L. Aqua-acupuncture Solution (PVAS), PhaseⅠenzyme, PhaseⅡenzyme, DNA adduct
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