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KMID : 1129720020190010079
Korean Journal of Acupuncture
2002 Volume.19 No. 1 p.79 ~ p.86
Shenqi-wan Increases Cell Proliferation of Cultured Hippocampal
Kim Youn-Jung

Jang Mi-Hyeon
Shin Min-Chul
Lim Baek-Vin
Chung Joo-Ho
Bahn Geon-Ho
Paik Eun-Kyung
Park Jae-Hyung
Kim E-Hwa
Kim Chang-Ju
Abstract
Neurogenesis comprises of cell proliferation, survival and differentiation. Although neurogenesis ceases in most areas at birth, stem cells continue to generate neurons within the subventricular zone and hippocampal dentate gyrus throughout adult life1-6). Cell birth and neurogenesis have also been demonstrated in the dentate gyrus of the several mammals, including humans7,8). Previous studies have shown that several factors such as glucocorticoids, estrogen, N-methyl-d-aspartate (NMDA) receptor antagonist, serotonin, aging, seizures, and environmental stimuli influence the proliferation of granular cell precursors and/or neurogenesis in the adult dentate gyrus9-15). Proliferating cells can be identified using 5-bromo-2-deoxyuridine (BrdU), which labels cellular DNA during the synthesis, or S-phase7,10,16). Recent evidences suggest that cell proliferation and neurogenesis may play a role in learning and the hippocampal formation is clearly recognized as being involved in learning and memory13,15,17). Although cell proliferation and neurogenesis is not the only mechanism in either learning or recovery from damage, may be involved in learning in many cases13,15,17,18). Post-mitotic fate of a population of constitutively proliferating cells has been shown to be cell death19) or neuronal differentiation after migration20). It is known that growth factors promote the migration of proliferated cells into adjacent tissues where they differentiated21,22) and to begin generate neurons23,24). Recently, Aberg et al.25) showed that peripheral infusion of insulin-like growth factor-1 (IGF-1), a growth-promoting peptide hormone, increases progenitor cell proliferation and selectively induces neurogenesis in the progeny of adult neuronal progenitor cells in rat hippocampus. Traditionally, Shenqi-wan (SW), an Oriental herbal medicine formulation, has been used for delayed mental and physical development of children and complications of diabetes26,27). To study the medications which possess the ability of neuronal stem cells to differentiated into neurons indigenous to various brain regions offers hope for improve mental retardation and/or restorative therapies for ischemia, traumatic and degenerative brain diseases. However, there are to our knowledge no known exogenous herbal substances that selectively increase cell proliferation in the mammalian central nervous system (CNS). In this study, ability of SW which has been used for mental and physical retardation of children in Oriental medicine to confer increase of hippocampal cell proliferation was investigated.
KEYWORD
Shenqi-wan, MTT assay, BrdU immunohistochemistry, cell proliferation Cell Line HiB5 and Dentate Gyrus of Young Sprague-Dawley Rats
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