KMID : 1129720040210020125
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Korean Journal of Acupuncture 2004 Volume.21 No. 2 p.125 ~ p.137
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Effects of Chelidonii Herbal-acupuncture solution Anti-inflammatory in RAW 264.7 macrophages
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Park Dong-Cheon
Park Ji-Hyeon Lee U-Kyung Leek Jin-Kyu Seo Il-Bok Kim Ho-Hyun Kim Jung-Sun Kim E-Hwa
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Abstract
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Objectives : Recently, Herbal-acupuncture therapeutics has been used for the treatment of inflammatory diseases such as rheumatoid arthritis. Especially, we have been interested in chemical mediators concerned with inflammation such as prostaglandin, cytokine, nitrous oxide. The purpose of this study is investigated that the effect of Chelidonii Herbal-acupuncture solution in lipopolysaccharide-stimulated RAW 264.7 macrophages, performed several expeimental items : those are prostaglandin E2, nitric oxide and cyclooxygenase-2.
Methods : The cytotoxicity of Chelidonii Herbal-acupuncture solution in RAW 264.7 macrophages were measured by MTT-based cytotoxicity assay. In order to observe cyclooxygenase-2 mRNA expression in lipopolysaccharide-stimulated RAW 264.7 macrophages, RT-PCR was used. Prostaglandin E2 formation and nitric oxide production was measured by competitive enzyme immunoassay and Griess assay.
Results : 1.The MTT assay demonstrated that cytotoxic effect of Chelidonii Herbal-acupuncture solution in RAW 264.7 macrophages were not appeared before concentration of 1§·/§¢. 2.Chelidonii Herbal-acupuncture solution inhibited cyclooxygenase-2 mRNA expression in lipopolysaccharide-stimulated RAW 264.7 macrophages. 3.Chelidonii Herbal-acupuncture solution inhibited nitric oxide production in lipopolysaccharide-stimulated RAW 264.7 macrophages. 4.Chelidonii Herbal-acupuncture solution inhibited prostaglandin E2 formation in lipopolysaccharide-stimulated RAW 264.7 macrophages.
Conclusions : On the basis of these results, It was shown that Chelidonii Herbal-acupuncture solution is significantly able to inhibit the production of PGE2 and NO, as well as COX-2 mRNA expression. Our results may provide new mechanism by which Chelidonii Herbal-acupuncture solution accounts for its beneficial effect on accelerating wound healing and anti-inflammation.
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KEYWORD
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Chelidonii Herbal-acupuncture, Anti-inflammatory, RAW 264.7 macrophages, PGE2 & NO, COX-2, mRNA expression
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