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KMID : 1129720050220010117
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Korean Journal of Acupuncture
2005 Volume.22 No. 1 p.117 ~ p.132
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Effect of Juglandis Semen Herbal Acupuncture on Toxic Agent-Induced Liver Cell Damage
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Lee Kyung-Tae
Kim Cheol-Hong
Youn Hyoun-Min
Jang Kyung-Jeon
Ahn Chang-Beohm
Song Chun-Ho
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Abstract
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Objectives : This study was carried out to determine whether Juglandis Semen herbal acupuncture (JSA) exerts the protective effect against toxic agent-induced live. cell damage.
Methods : The cell damage was estimated by measuring lactate dehydrogenase (LDH) release, and lipid peroxidation was estimated by measuring maiondialdehyde (MDA), a product of lipid peroxidation, in rabbit liver slices.
Results : When tissues were incubated with 0.5 mM Hg for , LDH release and lipid peroxidation were increased as a function of incubation time, and these effects were significantly prevented by addition of 0.1% JSA. Hg increased LDH release and lipid peroxidation in dose-dependent manner over the range of concentrations, which were reduced by 0.1% JSA. When tissues were treated with 0.5 mM Hg in the presence of JSA, LDH release and lipid peroxidation induced by Hg were prevented by JSA in a dose-dependent fashion. JSA at 0.5 and 1% prevented completely effects of 0.5 mM Hg. When tissues were treated with 0.5 mM Hg for 60 min, LDH release and lipid peroxidation were increased, which were significantly prevented by addition of 0.1 % JSA. tert-Butyl hydroperoxide (tBHP) increased LDH release and lipid peroxidation, which were significantly reduced by 0.1 % JSA. Such protective effects were similar to those of N,N’-diphenyl-p-phenylenediamine (DPPD), a potent antioxidant. When tissues were treated with 0.5 mM Hg, activities of catalase and glutathione peroxidase were inhibited, and glutathione content was also reduced. Such effects were prevented by JSA, but not by DPPD. JSA prevented Hg-induced morphological changes.
Conclusions : These results indicate that JSA exerts the protective effect against liver cell injury induced by toxic agents through antioxidant action, and this effect may be attributed to an increase in activities of endogeous anitoxidant enzymes and GSH content. However, antioxidant effect of JSA is different from that of a well-known potent antioxidant DPPD.
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KEYWORD
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Juglandis Semen, Herbal acupuncture, Liver cell damage, LDH, MDA, tBHP, DPPD
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