KMID : 1129720050220040057
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Korean Journal of Acupuncture 2005 Volume.22 No. 4 p.57 ~ p.65
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Antioxidant Property of Aqua-Acupuncture Solution from Circium japonicum
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Lee Jeong-Joo
Moon Jin-Young
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Abstract
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Objectives : Circium japonicum is a pharmacologically active used in traditional Korean medicine. An aqua-acupuncture solution of the Circium japonicum was assessed to determine the mechanism of its antioxidant activity.
Materials : Circium japonicum was obtained from a Dongguk Korean Medicine Hospital (Kyung-ju, Kyungbuk). The freeze-dry powder was collected (yield 5.1%) for the aqua-acupuncture solution. Scavenging activity on DPPH free radicals by the Circium japonicum aqua-acupuncture solution (CJAS) was assessed according to the method followed by Gyamfi et al.. and then scavenging activity orl superoxide radicals was assessed by the method described by Gotoh et al. with slight modification. Deoxyribose assay to determine the rate constant for the reactions between either antioxidants and hydroxyl radicals or antioxidants and iron ions. We tested by; (1) Non-site-specific scavenging assay (hydroxyl radicals, OH), (2) Site-specific scavenging assay (chelate iron ions), and (3) Pro-oxidant effect of the CJAS on iron dependent hydroxyl radical generation. Finally, we determined hydroxyl radical-mediated DNA nicking formation.
Conclusion : Our study demonstrated that CJAS has antioxidant activities and we investigated the potential effectiveness of CJAS in preventing oxidative stress-mediated disease further.
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KEYWORD
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aqua-acupuncture solution, antioxidant, oxidative stress, hydroxyl radicals, DNA nick formation
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