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KMID : 1129720080250010229
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Korean Journal of Acupuncture
2008 Volume.25 No. 1 p.229 ~ p.246
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Anti-apoptotic and neuroprotective effects of acupuncture techniques of tonification or sedation at HT9LR1, HT3KI10 on focal brain ischemic injury induced by intraluminal filament insertion in rats
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Byun Jeng-Yun
Youn Dae-Hwan
Na Chang-Su
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Abstract
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Objectives: Acupoints of LR1(Dadun)HT9(Shaochong), KI10(Yingu)HT3(Shaohai) are used as controlling the diseases from heart problems. Especially, when they are used, tonification or sedation techniques method is taken as a controlling the medication for an early stroke in the Korean medicine. So the aim of this study is to investigate the anti-apoptotic and neuroprotective effects of Acupuncture techniques of tonification or sedation at LR1HT9, KI10HT3 on the focal ischemia induced by intraluminal filament insertion in rats.
Materials and Methods: The focal ischemia was induced by intraluminal filament insertion into middle cerebral artery. The animals were divided into five groups (n=7 in each group) : Control group, no therapy group after ischemia induced; AT-1; acupuncture therapy group at LR1HT9, KI10HT3 after ischemia-induced. AT-2; acupuncture therapy at right LR1, HT9(forward direction), KI10HT3(opposite direction) inserting to the direction of route of the each meridian. AT3; acupuncture therapy at right LR1, HT9(twirling forward with the thumb of right hand 9 times), KI10HT3(twirling forward with the forefinger of right hand 6 times) AT-4; acupuncture therapy at right LR1, HT9(forward direction, twirling forward with the thumb of right hand 9 times), KI10HT3(opposite direction, twirling forward with the forefinger of right hand 6 times) inserting to the direction of route of the each meridian. The anti-apoptotic and neuroprotective effects of Acupuncture techniques of tonification or sedation at LR1, HT9, KI10HT3 were observed by Bax, Bcl-2, mGluR5, Cytochrome c, Cresyl violet and ChAT-stain.
Results: The intensity of Bax and Bax/Bcl-2 ratio were increased in ACU-2 and ACU-4 group but decreased in ACU-3 group. The intensity of mGluR5 was increased in ACU-1, ACU-2, ACU-3 and ACU-4 group. The intensity of cytochrome c was decreased in ACU-3 and ACU-4 group. The density of neurons stained by Cresyl violet and ChAT were increased in ACU-1, ACU-3 and ACU-4 group.
Conclusions: Our study suggests that acupuncture therapy of tonification at LR1, HT9 by twirling forward with the thumb of right hand 9times, sedation at KI10HT3 by twirling forward with the forefinger of right hand 6 times after perpendicularly inserting needle shows anti-apoptotic and neuroprotective effects on cholinergic neuron in focal cerebral ischemia of the stroke in rats.
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KEYWORD
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Ischemia, Acupuncturetechniques, LR1(Dadun), HT9(Shaochong), KI10(Yingu), HT3(Shaohai)
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