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KMID : 1129720100270010031
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Korean Journal of Acupuncture
2010 Volume.27 No. 1 p.31 ~ p.47
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Immunomodulatory activity of cultivated wild ginseng pharmacopuncture
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Kim Young-Jin
Lee Joon-Moo
Lee Eun
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Abstract
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Objectives: To investigate the anti-inflammatory effects of cultivated wild ginseng pharmacopuncture in lipopolysaccharide (LPS)-induced inflammatory rat model.
Methods: Sprague-Dawley rats were divided into 4 groups; LPS control (n=6), LPS+cultivated wild ginseng pharmacopuncture at CV4 (n=6), LPS+cultivated wild ginseng pharmacopuncture at CV17 (n=6), and LPS+cultivated wild ginseng pharmacopuncture at Ex-HN1 (n=6). Pharmacopuncture (0.1 ㎖) was given every two days for 4 weeks followed by inflammation induction by peritoneal LPS injection (5 ㎎/㎏). Blood, liver tissue, and peritoneal lavage fluid were taken and proinflammatory cytokines and other related factors were analysed.
Results: Compared with the control group, CV4 and Ex-HN1 pharmacopuncture groups significantly attenuated plasma IL-1β, IL-6, and TNF-α increase at 2h and 5h after LPS injection (P<0.05). A significant difference from control group emerged at 5 h for plasma IL10 (P<0.05). For liver cytokines analyzed at 5 h after LPS injection, only CV4 pharmacopuncture group showed significant difference in TNF-α and IL-10 (P<0.05). Blood CD4/CD8 ratio and the phagocytic activities of polymorphonuclear neutrophils were not different from those of control group in all pharmacopuncture groups (P>0.05). CV4 pharmacopuncture significantly attenuated increase of plasma NO3-/NO2-, Intracellular adhesion molecule-1 (ICAM-1), cytokine-induced neutrophil chemoattractant-1 (CINC-1), and prostaglandin E2 (PGE2) compared with the control group (P<0.05). Monocyte chemoattractant protein-1, PGE2, and CINC-1 level of CV4 pharmacopuncture group was significantly different from those from the control group (P<0.05).
Conclusions: These results indicate that cultivated wild ginseng pharmacopuncture at CV4 may have a potent anti-inflammatory effect in an LPS-induced inflammatory rat model.
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KEYWORD
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pharmacopuncture, cultivated wild ginseng, anti-inflammation, CV4
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