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KMID : 1129720100270010125
Korean Journal ofAcupuncture
2010 Volume.27 No. 1 p.125 ~ p.142
Comparative studies of effects of manual acupuncture, invasive laser acupuncture and laser skin irradiation at the Heart Jeonggyeok acupoint on the focal ischemia induced by intraluminal filament insertion in rats
Youn Jeong-Young

Youn Dae-Hwan
Lee Suk-Hee
Oh Gwang-Hwan
Jeong Sung-Ho
Na Chang-Su
Kim Young-Sun
Abstract
Objectives: The purpose of this study was to identify the effectiveness of neuronal activities for the acupuncture and laser acupuncture application.

Methods: The subject were divided into 7 groups as control group without acupuncture, acupuncture treatment with tonify manipulation with the direction of channel at HT9, LR1(AT-A), acupuncture treatment with purge manipulation against the direction of channel at HT3, Kl10(AT-B), acupuncture treatment with tonify manipulation with the direction of channel at HT9, LR1 and purge manipulation against the direction of channel at HT3, KI10(AT-C), laser acupuncture treatment with red light 658 nm at HT9, LR1(LAT-A), laser acupuncture treatment with green light 532 nm at HT3, KI10(LAT-B), laser acupuncture treatment with red light 658 nm at HT9, LR1 and green light 532 nm at HT3, KI10(LAT-B). Antiapopotic effect of acupuncture was observed by Bax, Bcl-2 and cytochrome C. Neuroprotective effect of acupuncture was observed by cresyl violet and ChAT.

Results: AT-A, AT-B, AT-C, LAT-A, LAT-B and LAT-C groups were significantly increased comparing the control groups in expression ChAT and in neuroprotective effect by cresyl violet. AT-A, AT-B, AT-C, LAT-A, LAT-B and LAT-C groups were significantly decreased comparing the control groups in expression Bax. AT-C, LAT-A, LAT-B and LAT-C groups were significantly increased comparing the control groups in expression Bcl-2. AT-A, AT-B, AT-C, LAT-A, LAT-B and LAT-C groups were significantly decreased comparing the control groups in Bax/Bcl-2 ratio. LAT-B and LAT-C groups were significantly decreased comparing the control groups in expression cytochrome C.

Conclusions: The acupuncture with tonify and purge manipulation and laser acupuncture with red and green light could be effective for antiapopotic and neuroprotective effect in focal brain ischemia.
KEYWORD
Antiapoptotic effect, Neuroprotective effect, Focal brain ischemia, Acupuncture, Laser acupuncture, Heart Jeonggyeok
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학술진흥재단(KCI)