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KMID : 1129720100270020013
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Korean Journal of Acupuncture
2010 Volume.27 No. 2 p.13 ~ p.24
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Antioxidative and Anti-inflammatory Effects of Aurantii Fructus Immaturus Pharmacopuncture
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Kim Seong-Jin
Park Sang-Kyun
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Abstract
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Objectives : Ulcerative colitis is a chronic relapsing inflammatory disease in the gastrointestinal tract. We investigated whether Aurantii fructus immaturus (AFI) pharmacopuncture has antioxidative and anti-inflammatory effects.
Methods : in vitro experiments, 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging activity, superoxide dismutase (SOD) activity, prevention on H2O2-induced cell death in RAW264.7 cell line, DNA fragmentation, and cyclooxygenase-2 mRNA expression induced by lipopolysaccharide (LPS), were analyzed to investigate antioxidative and anti-inflammatory effect of AFI pharmacopuncture. in vivo experiment, a murine model of dextran sulfate sodium (DSS)-induced colitis was used to examine the effect of AFI pharmacopuncture on CV12 at different doses of 5 ㎕, 0.5 ㎕, 0.05 ㎕ for 10 days. Body weight, colon length and macroscopic features were investigated.
Results : AFI pharmacopuncture showed DPPH free radical scavenging and SOD active effects in a dose-dependent manner. AFI pharmacopuncture showed a protective effect against H2O2 -induced cell injury and also attenuated LPS-induced COX-2 mRNA expression. In a DSS- induced colitis murine model, however, AFI pharmacopuncture at CV12 had no anti-inflammatory effects.
Conclusions : The present results suggest that AFI pharmacopuncture extract may have anti- inflammatory and antioxidative effects in vivo test, but further research on the underlying mechanism is required.
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KEYWORD
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Aurantii fructus immaturus, Antioxidative, Anti-inflammation, Dextran sulfate sodium, Inflammatory bowel disease
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