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KMID : 1129720110280030043
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Korean Journal of Acupuncture
2011 Volume.28 No. 3 p.43 ~ p.51
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Effect of Fructus Ligustri Lucidi H2O Extract on Cell Proliferation in Hman Dermal Fibroblast
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Lim Nan-Young
Kim Dae-Sung
Ko Kyoung-Sook
Mun Yeun-Ja
Woo Won-Hong
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Abstract
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Objectives: In this study, we investigated the effect of Fructus Ligustri Lucidi fraction (FLLW) on cell proliferation, and the phosphorylation of ERKs and Akt in human dermal fibroblast neonatal (HDFn).
Methods: After treatment of HDFn with FLLW, MTT assay was performed to quantitatively determine cellular viability. The ERK and Akt pathways were analyzed in vitro by Western blot in a HDFn. HDFn proliferation after FLLW and minoxidil treatment in the absence or presence of PD98059, a MEK inhibitor, LY294002, and a PI3K inhibitor, was examined by Western blot or MTT assay.
Results: FLLW increased cell proliferation in a dose-dependent manner and minoxidil used as positive control also induced cell proliferation in HDFn. FLLW increased the phosphorylation of ERK and Akt. In addition, minoxidil, too, induced the phosphorylation of ERK and Akt in HDFn. PD98059 and LY294002 significantly attenuated FLLW-inducible p-ERK and p-Akt expression and proliferation in cultured HDFn.
Conclusions: Our results suggest that FLLW stimulates the growth of fibroblast cells through ERK and Akt pathways. Therefore, FLLW is a potential agent for the inducer of fibroblast growth.
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KEYWORD
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Human Dermal Fibroblast cells, Fructus Ligustri Lucidi, minoxidil
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