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KMID : 1129720130300040212
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Korean Journal of Acupuncture
2013 Volume.30 No. 4 p.212 ~ p.219
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The Anti-Migratory Effect of Cirsium japonicum Pharmacopuncture in C6 Glioma Cell
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박주연:Park Ju-Yeon
이강파:Lee Kang-Pa/장해룡:Chang Hae-Ryong/문진영:Moon Jin-Young
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Abstract
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Objectives: Cirsium japonicum is a traditional Korean medicine that has been used in the treatment of inflammatory diseases such as appendicitis, hepatitis, pulmonary abscess and tumor. The aim of study was to elucidate anti-migratory activity of CJP(Cirsium japonicum pharmacopuncture) through regulation of inflammatory mediators in C6 glioma cell.
Methods: Nitric oxide(NO) production was determined by using nitrite assay. The cell migration was analyzed by wound-healing assay and Boyden chamber assay. The expression levels of iNOS, and protein kinase C(PKC)-α were measured by western blotting assay.
Results: CJP showed a significant decrease on NO production. Moreover, glioma cell migration was effectively suppressed by CJP. Furthermore, CJP inhibited the expressions of iNOS and PKC-α in C6 glioma cells.
Conclusions: These results suggest that CJP inhibits glioma cell migration and iNOS expression through regulation of PKC-α. Therefore, it is expected that CJP could be an effective agents for blocking malignant progression of glioma.
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KEYWORD
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cirsium japonicum, migration, iNOS, PKC-α, C6 glioma cells
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