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KMID : 1129720140310010014
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Korean Journal of Acupuncture
2014 Volume.31 No. 1 p.14 ~ p.19
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Inhibitory Effects of Syzygium aromaticum Ethanol Extracts on IgE Mediated RBL-2H3 cell Activation
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정준희:Jung Jun-Hee
김용민:Kim Yong-Min/박종필:Park Jong-Phil/김태연:Kim Tae-Yeon/김이화:Kim Ee-Hwa
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Abstract
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Objectives : In this report, we investigated the effect of ethanol extract of Syzygium aromaticum(L.) Merr. & Perry.(SAE) on the RBL-2H3 cell-mediated allergic response and studied its possible mechanisms of action.
Methods : Cytotoxicity on RBL-2H3 cell was evaluated by MTT assay. Anti-allergic activity of SAE was assessed by β-Hexosaminidase and Histamine secretion, β- Hexosaminidase and Histamine secretion were measured by ELISA assay. Evaluate the mechanisms of effect of SAE on the secretion of degranulate mediators, we examined the effect of SAE on the activation of mitogen-activated protein kinases using western blot analysis.
Results : SAE had no cytotoxicity on rat basophilic leukemia cell(RBL-2H3). Moreover SAE dose-dependently inhibited RBL-2H3 cell degranulation and histamine release. SAE specifically blocked the IgE-induced p38 mitogen-activated protein kinase activation.
Conclusions : Our findings provide evidence that Syzygium aromaticum ethanol extract inhibits mast cell derived allergic reaction, and also demonstrate the involvement of p38 MAPK phosphorylation.
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KEYWORD
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Syzygium aromaticum, anti-allergic, mitogen-activated protein kinase
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