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KMID : 1129720150320020051
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Korean Journal of Acupuncture
2015 Volume.32 No. 2 p.51 ~ p.58
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Pinus densiflora Gnarl Inhibits Migration through Suppression of Protein Kinase C in C6 Glioma Cells
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민일국:Min Il-Guk
이강파:Lee Kang-Pa/장해룡:Chang Hae-Ryong/문진영:Moon Jin-Young
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Abstract
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Objectives: Pinus densiflora gnarl, called Song-Jeol in Korean medicine, has been used to cure inflammatory diseases such as arthritis. In the present study, we evaluated inhibitory property of Song-Jeol pharmacopuncture(SJ) on C6 glioma cell migration.
Methods: To evaluate cell viability on C6 glioma cells of SJ, the viability was assessed by using Ez-cytox assay kit. The cell migration was assessed by wound-healing assay and Boyden chamber assay, respectively. LPS-induced NO productions were determined by using the Griess reagent. The expression of iNOS and protein kinase C(PKC)-α were estimated by western blotting assay.
Results: In the wound-healing assay and Boyden chamber assay, SJ showed a significant inhibition on serum-induced C6 glioma cell migration. In addition, NO production was decreased by SJ through suppression of iNOS expression in LPS-stimulated C6 glioma cell. Futhermore, LPS-induced protein kinase C(PKC)-α expression was effectively inhibited by SJ.
Conclusions: These results demonstrated that SJ was useful for the suppression of the C6 glioma cell migration.
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KEYWORD
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Pinus densiflora gnarl, migration, iNOS, protein kinase C, C6 glioma cells
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