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KMID : 1129720150320040169
Korean Journal of Acupuncture
2015 Volume.32 No. 4 p.169 ~ p.176
The Anti-inflammatory Effect and DPPH Free Radical Scavenging Capability of Rhizoma drynariae Aqueous Extract
±è¿ë¹Î:Kim Yong-Min
±èÀÌÈ­:Kim Ee-Hwa
Abstract
Objectives: This study is to investigate the effects of Rhizoma drynariae aqueous extract(RDA) on cell cytotoxicity, Nitric Oxide (NO) and Prostaglandin E2(PGE2) production and 1,1-diphenyl-2-picryl ghdrazyl(DPPH) free radical scavenging capability.

Methods: Cell cytotoxicity was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay. The production of NO was measured by Griess assay. The production of PGE2 was measured by immunoassay. And, the anti-oxidant activity was measured by the DPPH method.

Results: Cell cytotoxicity in 50, 100, 200 and 400 ¥ìg/ml RDA did not increase significantly compared to the RDA untreated group. RDA(200¥ìg/ml and 400 ¥ìg/ml) inhibited NO and PGE2 production in lipopolysaccharide-stimulated RAW 264.7 cells. RDA had high DPPH free radical scavenging capability.

Conclusions: This study indicates that RDA inhibits NO and PGE2 production in lipopolysaccharide-stimulated RAW 264.7 cells and improve DPPH free radical scavenging capability. RDA may have an anti-inflammation effect and an anti-oxidant activity.
KEYWORD
anti-inflammation, anti-oxidant activity, Nitric Oxide, Prostaglandin E2, Rhizoma drynariae
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