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KMID : 1129720160330040204
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Korean Journal of Acupuncture
2016 Volume.33 No. 4 p.204 ~ p.212
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Effects of Direct Moxibustion Applied to EX-LE4 and EX-LE5 on the Pain Behavior and Expression of TRPM8 in the Rat Model of Ambient Cold Exposed Osteoarthritis
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Ji Byeong-Uk
Kim Yi-Quot
Lee Ji-Eun
Koo Sung-Tae
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Abstract
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Objectives: The aim of the study is to investigate the effects of moxibustion on the pain behavior and expression of TRPM8 in the dorsal root ganglion(DRG) in the rat model of ambient cold(AC) exposed osteoarthritis(OA).
Methods: OA was induced by the injection of 50 μl of 2% monosodium iodoacetate(MIA) into the knee joint cavity. To examine the level of pain, weight bearing forces(WBFs) of affected limb was measured. For the AC exposure, the animals were housed in 6 h/day at 4°C for 14 days after MIA injection. Moxibustion treatment was performed at EX-LE4 and EX-LE5 with 5 cons(1, 7 or 10 mg) per day for 13 days from 5 days after MIA injection. The expressions of TRPM8 in DRG were measured by western blotting analysis.
Results: The WBFs of MIA-AC group were decreased significantly compared to MIA group at 2, 3, 6, 7, 8 and 9 days after arthritis induction. After the first 6 h-AC exposure, expressions of TRPM8 in MIA-AC group were increased significantly compared to those of naive group. After moxibustion treatment, only the WBFs of 7 mg treated group were restored significantly. Moreover, the over-expressions of TRPM8 were attenuated by the moxibustion treatment in AC exposed rats.
Conclusions: The data suggest that AC can increase arthritic knee pain via up-regulated TRPM8 and moxibustion treatment improve the arthritic pain via modulation of TRPM8 expression in DRG in the rat model of AC exposed MIA induced arthritis.
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KEYWORD
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monosodium iodoacetate induced arthritis, ambient cold, TRPM8 channel, EX-LE4, EX-LE5, moxibustion
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