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KMID : 1129720170340020082
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Korean Journal of Acupuncture
2017 Volume.34 No. 2 p.82 ~ p.87
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The Effect of Lactobacillus Mixture Culture Fluid Extracts on Atopic Dermatitis Chemokine Expression of in HaCaT Cells
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Hong Soo-Jeong
Lee Won-Jae
Jo Eul-Hwa
Ahn Seong-Hun
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Abstract
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Objectives: Recently the case of lactobacillus mixture culture fluid appliment was reported. In this study, anti-inflamation effects and anti-allergy effects were studied by stimulus of lactobacillus mixture culture fluid extracts in HaCaT cells.
Methods: The atopic dermatitis were induced by TNF-α and interferon-γ in HaCaT cells. TARC/CCL17, MDC/CCL22, RANTES/CCL5 and ROS production were investigated to explain anti-inflamation and allergy effects of lactobacillus mixture culture fluid with cell-enzyme?linked Immunosorbent assay in 450 nm, 485 nm, 535 nm with spectro-fluorometer.
Results: The extracts of lactobacillus mixture culture fluid were decreased TARC/CCL17, MDC/CCL22, RANTES/CCL5 expressions and ROS production with a concentration dependent manner.
Conclusions: The effects mechanism of Lactobacillus mixed culture fluid for atopic dermatitis symptoms were considered to be explain anti-inflamation and allergy effects via control of cytokine, chemokine and ROS production, and the fluid could be applied in skin cells directly. But classified AD symptom degrees reported in clinical case before as Reaction Period, Reduction Period, Effect Period, Reproduction Period and Rebound Period could not be explained. Further study will be expected.
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KEYWORD
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atopic dermatitis, lactobacillus mixted culture fluid, HaCaT cell, TARC/CCL17, MDC/CCL22, RANTES/CCL5
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