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KMID : 1129720180350020091
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Korean Journal of Acupuncture
2018 Volume.35 No. 2 p.91 ~ p.97
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Effects of the Pharmacopuncture with MOK on Immune Regulation by Th1/Th2 Cytokines in L-Thyroxine-Induced Hyperthyroid Rats
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Hwang Ji-Hye
Jung Hyo-Won
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Abstract
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Objectives : This study was performed to investigate immune regulatory effects of the pharmacopuncture with MOK on hyperthyroid rats.
Methods : The experimental hyperthyroidism was prepared by the intraperitoneal injection of L-thyroxin (LT4, 0.5 mg/kg) once daily for 2 weeks in Sprague-Dawley(SD) rats. The pharmacopuncture with MOK extrac (MOK pharmacopuncture) at doses of 0.3 or 3 mg/kg was injected on acupuncture points in the thyroid glands of hyperthyroid rats once a day for 2 weeks. Propylthiouracil(PTU, 10 mg/kg) as a reference group was subcutaneously injected into the dorsal neck. We measured the levels of IFN-γ and IL-4 in the sera of rats using enzyme-linked immunosorbant assay(ELISA) and determined the expression of IFN-γ, IL-4, IL-10, and Foxp3 in spleen tissues by reverse transcriptase-polymerase chain reaction(RT-PCR).
Results : The treatment of MOK pharmacopuncture in hyperthyroid rats significantly decreased the serum levels of Th1 cytokine, IFN-γ(p＜0.01 for MOK 0.3 mg/kg, p＜0.05 for MOK 3 mg/kg, and p＜0.05 for PTU) and significantly increased the levels of Th2 cytokine, IL-4(p＜0.05 for MOK 0.3 mg/kg, p＜0.001 for MOK 3 mg/kg, and p＜0.05 for PTU) compared to control group. Also, the MOK pharmacopuncture significantly increased IL-4 expression(p＜0.05 for MOK 3 mg/kg, and p＜0.05 for PTU), IL-10(p＜0.05 for MOK 3 mg/kg, and p＜0.01 for PTU), and Foxp3(p＜0.01 for MOK 0.3 mg/kg, p＜0.05 for MOK 3 mg/kg and p＜0.01 for PTU) in spleen tissues of hyperthyroid rats compared to control group.
Conclusions : Our results suggest that MOK pharmacopuncture can help to ameliorate the pathological progression of hyperthyroidism by regulation of the Th1/Th2 imbalance.
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KEYWORD
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pharmacopuncture, hyperthyroidism, immune regulation, Th1/Th2 cytokines, MOK
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