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KMID : 1129720180350040166
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Korean Journal of Acupuncture
2018 Volume.35 No. 4 p.166 ~ p.173
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The Effect of Woogakseungmatang Extract on NO Production in LPS- Stimulated RAW 264.7 Cells
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Jo Na-Young
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Abstract
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Objectives : Woogakseungmatang is a prescription medication mainly used to treat facial paralysis in Korean medicine. The purpose of this study is to investigate the effects of Woogakseungmatang on anti-inflammation and anti-oxidation.
Methods : Woogakseungmatang was extracted using hot water. Cytotoxicity was assessed using the 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide(MTT) method; nitric oxide(NO) production and Prostaglandin E2(PGE2) production in RAW cells treated with Woogakseungmatang were investigated; and the cytokine changes associated with inflammation were examined. The antioxidant capacity of Woogakseungmatang was measured using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method.
Results : RAW cells treated with Woogakseungmatang showed 90% cell viability at a 100-μg/ml concentration. NO production was decreased by 15% at a 100-μg/ml concentration. PGE2 production was decreased by 18% at a 100-μg/ml concentration. Interleukin 1β(IL-1β), interleukin 6(IL-6), and tumor necrosis factor-α(TNF-α) were significantly reduced at 100 μg/ml compared with those in the control group. The DPPH free radical scavenging capability was more than 50% at 100 μg/ml.
Conclusions : Woogakseungmatang showed only a slight anti - inflammatory effect at 100 μg/ml and it was difficult to confirm the concentration-dependent anti-inflammatory effect. Therefore, this study means to confirm the potential anti-inflammatory effects of Woogakseungmatang. Based on this research, more systematic and diverse studies should be conducted.
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KEYWORD
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Woogakseungmatang, Seogakseungmatang, Anti-inflammation, Antioxidant, Macrophage, Korean Medicine
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