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KMID : 1129720190360020115
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Korean Journal of Acupuncture
2019 Volume.36 No. 2 p.115 ~ p.126
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Forsythiae Fructus Extract Inhibits RANKL-Induced Osteoclast Differentiation and Prevent Bone Loss in OVX-Induced Osteoporosis Rat
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Eom Ji-Whan
Kim Jae-Hyun
Kim Min-Sun
Kim Sang-Woo
Shin Hwa-Jeong
Jung Hyuk-Sang
Sohn Young-Joo
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Abstract
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Objectives : Osteoporosis is a condition characterized by low bone mass and increased bone fragility. It has become a major problem of senior citizens. The purpose of this study is to experiment the effect of water extract of Forsythiae Fructus (wFF) on osteoclast differentiation; and the other purpose is to examine the effect of wFF on osteoporosis in ovariectomized rat.
Methods :To investigate the effect of wFF on osteoclast differentiation and activity, RAW 264.7 cells were used. The number of TRAP positive cell, TRAP activity, pit area, mRNA expression of makers (RANK, TRAP, CA II, CTK, MMP-9, NFATc1, c-Fos), protein expression of makers (NFATc1, c-Fos) were investigated. For in vivo study, 40 female Sprague-Dawley (SD) rats were induced osteoporosis by ovariectomy (OVX) and then tested for anti-osteoporosis effect by administration of wFF.
Results : wFF suppressed osteoclatogenesis, TRAP activity and pit area formation. Moreover, wFF decreased the expression of master differentiation factors (NFATc1, c-Fos) and also reduced the osteoclastogenesis-related markers (TRAP, CA II, CTK, MMP-9). These suggest that wFF inhibit osteoclasts differentiation and bone resorption. In the OVX rat model, wFF inhibited decreasing of BMD and trabecular area.
Conclusions : Forsythiae Fructus should be effective for osteoporosis prevention and treatment.
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KEYWORD
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Forsythiae Fructus, osteoporosis, osteoclastogenesis, RANKL, TRAP, ovariectomized-rat
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